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KMID : 1161420120150110955
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Journal of Medicinal Food
2012 Volume.15 No. 11 p.955 ~ p.958
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Antiproliferative Activity of Flower Hexane Extract Obtained from Mentha spicata Associated with Mentha rotundifolia Against the MCF7, KB, and NIH/3T3 Cell Lines
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Nedel Fernanda
Begnini Karine Rech
de Azambuja Carvalho Pedro Henrique
Lund Rafael Guerra
Beira Fatima T. A.
Del Pino Francisco Augusto B.
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Abstract
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This study assessed the antiproliferative effect in vitro of the flower hexane extract obtained from Mentha spicata associated with Mentha rotundifolia against the human breast adenocarcinoma (MCF-7), human mouth epidermal carcinoma (KB), and mouse embryonic fibroblast (NIH 3T3) cell lines, using sulforhodamine B (SRB) assay. A cell density of 2×104/well was seeded in 96-well plates, and samples at different concentrations ranging from 10 to 500?mg/mL were tested. The optical density was determined in an ELISA multiplate reader (Thermo Plate TP-Reader). Results demonstrated that the hexane extract presented antiproliferative activity against both the tumor cell lines KB and MCF-7, presenting a GI50 (MCF-7=13.09?mg/mL), TGI (KB=37.76?mg/mL), and IL50 (KB=291.07?mg/mL). Also, the hexane extract presented antiproliferative activity toward NIH 3T3 cells GI50 (183.65?mg/mL), TGI (280.54?mg/mL), and IL50 (384.59?mg/mL). The results indicate that the flower hexane extract obtained from M. spicata associated with M. rotundifolia presents an antineoplastic activity against KB and MCF-7, although an antiproliferative effect at a high concentration of the extract was observed toward NIH 3T3.
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KEYWORD
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antiproliferative assay, cancer cell lines, plant extracts, Mentha rotundifolia, Mentha spicata
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